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±Ù°ü¼¼Ã´Á¦·Î½á ¼­¾ç»ê °íÃß³ÃÀÌ(Horseradish, Armoracia rusticana) »Ñ¸® ÃßÃâ¹°ÀÇ Ç×±ÕÈ¿°ú

THE ANTIMICROBIAL EFFECT OF HORSERADISH (ARMORACIA RUSTICANA) ROOT EXTRACTS AS AN ENDODONTIC IRRIGANT

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À±È¿Áø ( Yun Hyo-Jin ) - °­¸ª¿øÁÖ´ëÇб³ Ä¡°ú´ëÇÐ ¼Ò¾ÆÄ¡°úÇб³½Ç
¹ÚÈ£¿ø ( Park Ho-Won ) - °­¸ª¿øÁÖ´ëÇб³ Ä¡°ú´ëÇÐ ¼Ò¾ÆÄ¡°úÇб³½Ç
ÀÌÁÖÇö ( Lee Ju-Hyun ) - °­¸ª¿øÁÖ´ëÇб³ Ä¡°ú´ëÇÐ ¼Ò¾ÆÄ¡°úÇб³½Ç
¼­Çö¿ì ( Seo Hyun-Woo ) - °­¸ª¿øÁÖ´ëÇб³ Ä¡°ú´ëÇÐ ¼Ò¾Æû¼Ò³âÄ¡°úÇб³½Ç

Abstract

Ä¡¼ö ¹× Ä¡±Ù´ÜÀÇ °¨¿°Àº ¹Ì»ý¹°¿¡ ÀÇÇØ ¾ß±âµÇ¸ç, ¼º°øÀûÀÎ ±Ù°üÄ¡·á´Â ±Ù°ü ³»ÀÇ ¹Ì»ý¹°ÀÇ ¼ö¸¦ °¨¼Ò½ÃÅ°´Â °Í¿¡ ÀÇÇØ Á¿ìµÈ´Ù. ¼­¾ç»ê °íÃß³ÃÀÌ(Horseradish, Armoracia ruticana)ÀÇ ÁÖ¼ººÐÀÎ Allyl isothiocyanate (AIT)´Â ¿©·¯ ¿¬±¸¿¡¼­ Ç×±ÕÈ¿°ú°¡ ÀÖÀ½ÀÌ º¸°íµÇ¾ú´Ù. ÀÌ¿¡ º» ¿¬±¸¿¡¼­´Â ±Ù°ü¼¼Ã´Á¦·Î½á ¼­¾ç»ê °íÃß³ÃÀÌ ÃßÃâ¹°ÀÇ Ç×±ÕÈ¿°ú¸¦ ¾Ë¾Æº¸°íÀÚ ¹ß°ÅµÈ »ç¶÷ÀÇ Ä¡¾Æ¿¡ Enterococcus faecalis¸¦ Á¢Á¾ÇÏ¿©, ´ëÇ¥ÀûÀÎ ±Ù°ü¼¼Ã´Á¦ÀÎ Â÷¾Æ¿°¼Ò»ê³ªÆ®·ý(NaOCl)°ú Ç×±Õ·ÂÀ» ºñ±³ÇÏ¿© ´ÙÀ½°ú °°Àº °á·ÐÀ»¾ò¾ú´Ù. 1. Ä¡Å«°ü ³»¸¦ 21ÀÏ°£ E. faecalis¿¡ °¨¿°½ÃÅ°°í, ±Ù°ü¼¼Ã´Á¦·Î½á °¢ ½ÇÇ豺À» 1ºÐ°£ Àû¿ëÇÑ ÈÄ 1Â÷ Ç¥º»(S1)À» äÃëÇÏ¿© ¹è¾çÇÑ °á°ú, ¼­¾ç»ê °íÃß³ÃÀÌ »Ñ¸® ÃßÃâ¹°Àº 5.815¡¿10©ø CFU/ml, NaOClÀº 3.465¡¿10©ø CFU/mlÀ̾ú´Ù(p=0.086). 2. 1Â÷ Ç¥º»À» äÃëÇÑ ÈÄ ½ÇÇè Ä¡¾Æ¸¦ 7ÀÏ µ¿¾È ´õ ¹è¾çÇÏ¿© 2Â÷ Ç¥º»(S2)À» äÃëÇÑ °á°ú ¼­¾ç»ê °íÃß³ÃÀÌ »Ñ¸® ÃßÃâ¹°Àº 3.100¡¿10©ø CFU/ml, NaOClÀº 5.252¡¿10^5 CFU/ml·Î, ¼­¾ç»ê °íÃß³ÃÀÌ »Ñ¸® ÃßÃâ¹°À» Àû¿ëÇÑ ½ÇÇè Ä¡¾Æ¿¡¼­ ´õ ÀûÀº ¼öÀÇ ¼¼±Õ¼ö°¡ ÃøÁ¤µÇ¾ú´Ù(p<.05). 3. NaOCl·Î ±Ù°ü¼¼Ã´À» ½ÃÇàÇÑ Ä¡¾ÆÀÇ 1Â÷ Ç¥º»(S1)°ú 2Â÷ Ç¥º»(S2)À» äÃëÇÏ¿© ¹è¾çÇÑ °á°ú 2Â÷ Ç¥º»¿¡¼­ CFU °ªÀÌ ´õ Áõ°¡ÇÏ¿´´Ù(p<.05). ¹Ý¸é¿¡ °íÃß³ÃÀÌ ÃßÃâ¹°·Î ±Ù°ü¼¼Ã´À» ½ÃÇàÇÑ Ä¡¾ÆÀÇ 1Â÷ Ç¥º»(S1)°ú 2Â÷ Ç¥º»(S2)À» äÃëÇÏ¿© ¹è¾çÇÑ °á°ú À¯ÀÇÇÑ Â÷ÀÌ´Â ¾ø´Â °Í(p=0.076)À¸·Î º¸¾Æ ±Ù°ü¼¼Ã´ÈÄ 7ÀÏ ÈÄ¿¡µµ Çâ±ÕÈ¿°ú°¡ Áö¼ÓµÊÀ» ¾Ë ¼ö ÀÖ¾ú´Ù.

Microorganisms are the main causative factors of pulpal and periapical diseases, therefore successful endodontic treatment is depend on the effective elimination of intracanal bacterial populations. Many studies have been reported antimicrobial effect of Allyl isothiocyanate (AIT) which the principle ingredient of Horseradish (Armoracia rusticana) root extracts. The purposes of this study are to evaluate the antimicrobial effectiveness of Horseradish root extracts against Enterococcus faecalis in root canals of extracted human teeth and compare to sodium hypochlorite (NaOCl). Extracted human mandibular premolar root canals were infected with E. faecalis for 21 days, and then irrigated with Horseradish root extracts, NaOCl solution and saline. After canal irrigation, first samples (S1) were taken. After first sampling, the canals were additionally incubated 7 days, and then second samples (S2) were taken. The samples were inoculated on EHI agar plate to determine the colony forming units (CFU). 1. Mean values of CFU in S1 were 5.815¡¿10©ø CFU/ml at Horseradish groups, and 3.465¡¿10©ø CFU/ml at NaOCI groups. There was no statistically significant differences (p=0.086). 2. Mean values of CFU in S2 were 3.100¡¿10©ø CFU/ml at Horseradish groups, and 5.252¡¿10^5 CFU/ml at NaOCI groups. There was statistically significant difference (p<.05). 3. There was no statistically significant differences (p=0.076) between S1 and S2 at Horseradish groups in the mean values of CFU. However, there was statistically significant differences (p<.05) between S1 and S2 at NaOCI groups in the mean values of CFU.

Å°¿öµå

¼­¾ç»ê °íÃß³ÃÀÌ »Ñ¸® ÃßÃâ¹°; Allyl isothiocyanate (AlT); ±Ù°ü¼¼Ã´Á¦; Enterococcus faecalis
Horseradish (Armoracia rusticana) root extracts; Allyl isothiocyanate (AlT); Endodontic irrigant; Enterococcus faecalis

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